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使用天然多酚姜黄素对脑组织淀粉样斑块进行标记和成像
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Labeling and Imaging of Amyloid Plaques in Brain Tissue Using the Natural Polyphenol Curcumin
DOI:

10:15 min

November 01, 2019

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Chapters

  • 00:05Title
  • 01:04Perfusion
  • 02:24Tissue Processing
  • 03:48Histochemical A-Beta Labeling of Cryostat Tissue Sections
  • 05:00Histochemical Labeling of Paraffin-Embedded Tissue Sections
  • 05:42A-Beta-Specific Antibody Labeling
  • 07:24Intracellular Amyloid-Beta Protein Colocalization
  • 08:00Results: Representative Curcumin-Amyloid Plaque Labeling and Imaging
  • 09:24Conclusion

Summary

Automatic Translation

姜黄素是一种理想的荧光素,用于标记和成像脑组织中的淀粉样贝塔蛋白斑块,因为它优先结合淀粉样贝塔蛋白,并且与其他传统的淀粉样蛋白结合染料的结构相似。与传统方法相比,它可用于更高效、更廉价地标记和图像淀粉样β蛋白斑块。

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