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Developmental Biology
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一种从人类胚胎干细胞中生成脑器官的静态自导方法
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Developmental Biology
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JoVE Journal
Developmental Biology
A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
一种从人类胚胎干细胞中生成脑器官的静态自导方法
DOI:
10.3791/60379-v
•
08:30 min
•
March 04, 2020
•
Erin M. Boisvert
,
Robert E. Means
,
Michael Michaud
,
Jason J. Thomson
,
Joseph A. Madri
,
Samuel G. Katz
1
Department of Pathology
,
Yale University School of Medicine
,
2
Stem Cell Center
,
Yale University School of Medicine
Chapters
00:05
Introduction
00:53
Stem Cell Maintenance
03:03
Dissociation of the hESCs for Organoid Culture
04:27
Generation of Organoids
06:06
Results: Overview of Organoid Growth Conditions and Morphology
07:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议是作为一种以简化、低成本的方式生产脑器官的方法,没有外源性生长因子或基底膜基质,同时仍然保持脑细胞类型的多样性和细胞组织的许多特征。
Tags
Brain Organoids
Human Embryonic Stem Cells
Neurodevelopmental Disorders
Toxic Insults
Brain Cancer
Reproducible
Simple Workflow
Cost-effective
Matrix
H9 HESCs
MTeSR1 Media
Low Oxygen Incubator
Protease Solution
Passaging
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