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Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy
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Developmental Biology
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JoVE Journal Developmental Biology
Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

DOI:
10.3791/63671-v

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11:02 min

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April 13, 2022

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, , ,
1Laboratory of Tissue Plasticity in Health and Disease, Cluster of Stem Cell and Developmental Biology, Department of Development and Regeneration, Leuven Stem Cell Institute,KU Leuven (University of Leuven), 2Biomedical Research Institute (BIOMED),UHasselt, Hasselt University

Chapters

  • 00:04Introduction
  • 01:16Dental Follicle Dissociation
  • 04:22Establishment of Tooth Organoid Culture
  • 06:11Amplification and Passaging of Tooth Organoid Culture
  • 08:47Results: Tooth Organoid Development, Characterization, and Validation
  • 10:14Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol to develop epithelial organoid cultures starting from human tooth. The organoids are robustly expandable and recapitulate the tooth's epithelial stem cells, including their ameloblast differentiation capacity. The unique organoid model provides a promising tool to study human dental (stem cell) biology with perspectives for tooth-regenerative approaches.

Tags

OrganoidsHuman ToothDental Stem CellsRegenerative TherapyDental TumorsEnamel FormationDental FolliclesDissociation

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