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使用微孔培养法生成具有睾丸特定结构的猪睾丸组织

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Medicine

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JoVE Journal Medicine

Generation of Porcine Testicular Organoids with Testis Specific Architecture using Microwell Culture

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使用微孔培养法生成具有睾丸特定结构的猪睾丸组织

DOI:

10.3791/60387-v

•

11:53 min

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October 03, 2019

•

Sadman Sakib, Yang Yu⁴, Anna Voigt, Mark Ungrin^3,4, Ina Dobrinski²

¹Biochemistry and Molecular Biology,University of Calgary, ²Comparative Biology and Experimental Medicine,University of Calgary, ³Biomedical Engineering Graduate Program,University of Calgary, ⁴Alberta Diabetes Institute,University of Alberta

Chapters

00:04Title
00:51Testis Tissue Enzymatic Digestion
05:22Germ Cell Enrichment
06:52Preparation of Cells and Microwells
08:21Generation of Testicular Organoids
09:51Results: Testis-specific Tissue Architecture
10:59Conclusion

Summary

Automatic Translation

在这里，我们提出了一个协议，用于使用市售的微孔培养系统，用睾丸特定组织架构的可重复生成猪睾丸器官。

Tags

Keywords: Porcine Testis Testicular Organoids Testis-specific Architecture Microwell Culture Testis Morphogenesis Drug Screening Toxicity Screening Enzymatic Digestion Collagenase DMEM Cell Isolation

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