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Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

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Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

DOI:

10.3791/60475-v

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07:57 min

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December 26, 2019

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Mukul Girotra*^1,2, Anne-Christine Thierry, Alexandre Harari³, George Coukos, Olaia Naveiras⁴, Nicola Vannini*¹

¹Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne,University of Lausanne, ²Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences,Swiss Federal Institute of Technology Lausanne (EPFL), ³Center of Experimental Therapeutics, Department of Oncology,Centre Hospitalier Universitaire Vaudois, ⁴Hematology Service, Department of Oncology,Centre Hospitalier Universitaire Vaudois (CHUV)

Chapters

00:04Title
01:59Ex Vivo Culture of FACS Sorted Hematopoietic Stem Cells
04:00Measurement of Mitochondrial Mass and Membrane Potential
06:21Results: Mitochondrial Profiles
07:18Conclusion

Summary

Automatic Translation

Here we describe a reliable method to measure mitochondrial mass and membrane potential in ex vivo cultured hematopoietic stem cells and T cells.

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Keywords: Mitochondrial Mass Mitochondrial Membrane Potential Flow Cytometry Hematopoietic Stem Cells T-cells Metabolic Assays Bone Marrow Transplantation Colony-forming Assays Immune T Cells Verapamil FACS Sorting LKS Population CD150 CD48 Stem Cell Expansion Medium

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