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遗传性痉挛性截瘫中人类诱导多能干细胞衍生神经元的线粒体迁移与形态分析

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia

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遗传性痉挛性截瘫中人类诱导多能干细胞衍生神经元的线粒体迁移与形态分析

DOI:

10.3791/60548-v

•

07:32 min

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February 09, 2020

•

Yongchao Mou², Sukhada Mukte, Eric Chai, Joshua Dein, Xue-Jun Li²

¹Department of Biomedical Sciences,University of Illinois College of Medicine Rockford, ²Department of Bioengineering,University of Illinois at Chicago, ³MD Program,University of Illinois College of Medicine Rockford

Chapters

00:04Introduction
00:50Telencephalic Glutamatergic Neuron Generation
01:49Time-Lapse Imaging of Mitochondrial Transport Along Telencephalic Glutamatergic Neuron Axons
02:44Cortical Neuron Mitochondrial Transport Analysis
04:17Cortical Neuron Mitochondrial Morphology Analysis
05:32Results: Representative Mitochondrial Transport and Morphology Analyses
06:47Conclusion

Summary

Automatic Translation

受损的线粒体迁移和形态学涉及各种神经退行性疾病。提出的协议使用诱导多能干细胞衍生前脑神经元来评估线粒体迁移和遗传性痉挛性截瘫的形态。该协议允许沿斧子对线粒体贩运进行表征，并分析其形态，这将有助于神经退行性疾病的研究。

Tags

Keywords: Mitochondrial Transport Mitochondrial Morphology Human Induced Pluripotent Stem Cell-derived Neurons Hereditary Spastic Paraplegia Axonal Degeneration Mitochondrial Dynamics Neurological Diseases Live Cell Imaging Mitochondrial Trafficking Therapeutic Targets Neurodegenerative Diseases Neurosphere Differentiation Axon Identification Mitochondrial Staining Time-lapse Imaging ImageJ Analysis

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