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斑马鱼胚胎和幼虫的整个山免疫性化学

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Developmental Biology

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JoVE Journal Developmental Biology

Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae

Please note that all translations are automatically generated. Click here for the English version.

斑马鱼胚胎和幼虫的整个山免疫性化学

DOI:

10.3791/60575-v

•

07:29 min

•

January 29, 2020

•

Dena R. Hammond-Weinberger, Gary T. ZeRuth

¹Department of Biological Sciences,Murray State University

Chapters

00:04Introduction
00:53Embryo Collection and Fixation
03:19Primary and Secondary Antibody Incubation
04:41Embryo Dissection, Deyolking, and Documentation
05:40Results: Whole Mount Immunohistochemistry
06:31Conclusion

Summary

Automatic Translation

在这里，我们提出了一个方案，荧光抗体介导检测蛋白质在斑马鱼胚胎和幼虫的整个制剂。

Tags

Whole Mount Immunohistochemistry Zebrafish Embryos Larvae Protein Expression Spatial And Temporal Confocal Microscopy 3D Representation Biomedical Research Disease Etiology Molecular Mechanisms Diagnostic Tool Tumor Identification Spawning Dechorionation Fixation Permeabilization Rehydration Methanol Storage

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