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Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.

Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.

DOI:
10.3791/60653-v

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09:06 min

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March 25, 2020

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, , , ,
1Department of Molecular Microbiology & Immunology,Brown University, 2Division of Surgical Research, Department of Surgery,Rhode Island Hospital and The Warren Alpert School of Medicine of Brown University

Chapters

  • 00:05Introduction
  • 00:53Subcutaneous Polyvinyl Alcohol (PVA) Sponge Implantation
  • 03:09PVA Sponge Fluid Isolation
  • 03:58PVA Sponge Cell Isolation
  • 05:33Tail Skin Excision
  • 06:57Results: Representative Assessment of Acute Wound Healing
  • 08:40Conclusion

Summary

Automatic Translation

Automatic Translation

Here, two murine wound healing models are described, one designed to assess cellular and cytokine wound healing responses and the other to quantify the rate of wound closure. These methods can be used with complex disease models such as diabetes to determine mechanisms of various aspects of poor wound healing.

Tags

Keywords: Wound HealingPVA Sponge ImplantationExcisional Tail Skin WoundCellular And Cytokine KineticsWound ClosureComorbid ConditionsDiabetesPneumoniaC57-Black/6 MouseSubcutaneous PocketPVA Sponge Implantation Procedure

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