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Genetics
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使用有条件Cas9稳定评估基因功能的新工具包
JoVE Journal
Genetics
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JoVE Journal
Genetics
A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
Please note that all translations are automatically generated.
Click here for the English version.
使用有条件Cas9稳定评估基因功能的新工具包
DOI:
10.3791/60685-v
•
08:20 min
•
September 02, 2021
•
Polona Šafarič Tepeš
2
,
Raffaella Sordella
1
Cold Spring Harbor Laboratory
,
2
Faculty of Pharmacy
,
University of Ljubljana
Chapters
00:04
Introduction
01:00
Lentiviral Preparation
02:47
Determining Virus Titer and Transduction Efficacy with Flow Cytometry
04:27
Lentiviral Transduction of Target Cells and Conditional Induction of Cas9 Mediated Gene Editing
06:06
Results: Analysis of Reversibility and Rapidity of Destabilized DD-Cas9 Protein
07:24
Conclusion
Summary
Automatic Translation
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在这里,我们描述了一个基因组编辑工具,基于时间和条件稳定聚类定期间歇性短腹膜重复(CRISPR-)相关蛋白质9(Cas9)下的小分子,盾牌-1。该方法可用于培养细胞和动物模型。
Tags
Keywords: CRISPR-Cas9
Gene Editing
Conditional Cas9 Stabilization
Shield-1
Essential Genes
Tumor Survival Genes
DD-Cas9
In Vivo
In Vitro
Blood-brain Barrier
HEK293T Cells
Transfection
Virus Supernatant
Virus Titer
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