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Forberedelse af perifere blod mononukleare celle pellets og plasma fra en enkelt blod draw på kliniske forsøg steder for Biomarkør Analyse
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
DOI:

07:40 min

March 20, 2021

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Chapters

  • 00:05Introduction
  • 01:08Whole Blood Irradiation for DNA Damage Response (DDR) Biomarker Expression Analysis
  • 03:35PBMC and Plasma Freezing
  • 04:45Western Blot Analysis
  • 05:43Results: Representative DNA Damage Assessment
  • 07:10Conclusion

Summary

Automatic Translation

Denne protokol beskriver klinisk implementerbar forberedelse af PBMC- og plasmabiossampler af høj kvalitet på det kliniske forsøgssted, der kan bruges til translationel biomarkøranalyse.

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