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冷冻组织部分多光谱荧光成像的快速方法
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
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冷冻组织部分多光谱荧光成像的快速方法

DOI:
10.3791/60806-v

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07:50 min

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March 30, 2020

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, , ,
1Robert Lurie Comprehensive Cancer Center and Department of Dermatology, Feinberg School of Medicine,Northwestern University, 2Department of Pathology,University of Illinois at Chicago, 3Department of Microbiology and Immunology, Feinberg School of Medicine,Northwestern University

Chapters

  • 00:05Introduction
  • 00:39Antibody and Fluorophore Selecting and Staining
  • 01:52Spectral Library Preparation
  • 02:59Multispectral Imaging
  • 03:54Spectral Unmixing
  • 04:37Analyzing Multispectral Images via Cell Segmentation and Phenotyping
  • 05:52Results: Representative Multispectral Imaging and Cell Quantification
  • 07:07Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一种快速染色方法，用于对冷冻组织进行多光谱成像。

Tags

Multispectral Fluorescence ImagingBiomarker DetectionCo-localizationRapid Staining MethodSpectral LibraryMachine LearningImage Analysis

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