Journal
/
/
联合非自然氨基酸合并和点击化学为疫苗目的生产的同质糖共酶
JoVE Journal
Bioengineering
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes
DOI:

13:53 min

December 19, 2020

, , , ,

Chapters

  • 00:00Introduction
  • 00:54Synthesis of the UAA: Propargyl-L-Lysine (Prk)
  • 04:46Production of the Recombinant Protein modified by PrK
  • 08:13Removal of the Histidine Tag by TEV Protease Digestion
  • 09:48Assessment of PrK Accessibility and Functionality and Conjugation to the Hapten
  • 11:26Results: Efficient Incorporation of the PrK and Click Conjugation
  • 12:49Conclusion

Summary

Automatic Translation

遗传代码扩展用于在定义位点载体蛋白上引入一种非自然氨基酸,该氨基酸具有生物角功能组。生物激素功能进一步用于碳水化合物抗原的位点选择性耦合,以提供均匀糖基结合疫苗。

Related Videos

Read Article