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Bioengineering
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联合非自然氨基酸合并和点击化学为疫苗目的生产的同质糖共酶
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Bioengineering
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JoVE Journal
Bioengineering
Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes
Please note that all translations are automatically generated.
Click here for the English version.
联合非自然氨基酸合并和点击化学为疫苗目的生产的同质糖共酶
DOI:
10.3791/60821-v
•
13:53 min
•
December 19, 2020
•
Typhaine Violo
,
Christophe Dussouy
,
Charles Tellier
,
Cyrille Grandjean
,
Emilie Camberlein
1
Université de Nantes, CNRS, Unité Fonctionnalité et Ingénierie des Protéines (UFIP), UMR 6286
Chapters
00:00
Introduction
00:54
Synthesis of the UAA: Propargyl-L-Lysine (Prk)
04:46
Production of the Recombinant Protein modified by PrK
08:13
Removal of the Histidine Tag by TEV Protease Digestion
09:48
Assessment of PrK Accessibility and Functionality and Conjugation to the Hapten
11:26
Results: Efficient Incorporation of the PrK and Click Conjugation
12:49
Conclusion
Summary
Automatic Translation
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Automatic Translation
遗传代码扩展用于在定义位点载体蛋白上引入一种非自然氨基酸,该氨基酸具有生物角功能组。生物激素功能进一步用于碳水化合物抗原的位点选择性耦合,以提供均匀糖基结合疫苗。
Tags
Keywords: Homogeneous Glycoconjugate
Vaccine
Unnatural Amino Acid
Click-chemistry
Genetic Code Expansion
Codon Suppression
Propargyl-lysine
Bioorthogonal Functional Group
N(alpha)Boc-propargyl-lysine
Synthetic Oligosaccharide Hapten
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