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Journal
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Genetics
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诱导毛茸茸的根由
农业细菌根
- 在塔特里巴克麦的中介转化 (
法戈皮鲁姆塔塔里库姆
)
JoVE Journal
Genetics
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JoVE Journal
Genetics
Inducing Hairy Roots by
Agrobacterium rhizogenes
-Mediated Transformation in Tartary Buckwheat (
Fagopyrum tataricum
)
Please note that all translations are automatically generated.
Click here for the English version.
诱导毛茸茸的根由
农业细菌根
- 在塔特里巴克麦的中介转化 (
法戈皮鲁姆塔塔里库姆
)
DOI:
10.3791/60828-v
•
08:12 min
•
March 11, 2020
•
Yaolei Mi
2
,
Zhihui Zhu
,
Guangtao Qian
,
Yu Li
,
Xiangxiao Meng
,
Jianping Xue
,
Qingfu Chen
,
Wei Sun
,
Yuhua Shi
2
1
Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica
,
China Academy of Chinese Medical Sciences
,
2
Artemisinin Research Center
,
China Academy of Chinese Medical Sciences
,
3
College of Life Science
,
Huaibei Normal University
,
4
Economic Crop Research Institute Sichuan Academy of Agriculture Sciences
,
5
Research Center of Buckwheat Industry Technology
,
Guizhou Normal University
Chapters
00:04
Introduction
01:01
Tartary Buckwheat (TB) Seed Preparation
01:49
TB Seedling and Explant Preparation
02:46
A. rhizogenes
Transformation Preparation
03:56
TB Explant Screening, Infection, and Co-Culture
04:47
Induction and Selective Culture and TB Hairy Root Subculture
05:32
Transformed Hairy Root Identification and Conservation
06:14
Results: Representative Hairy Root Transformation Identification and Characterization
07:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种通过
农业细菌根
诱导毛茸茸的根的方法 - 在塔特里麦(
法戈皮鲁姆塔塔里库姆
)中介导的转化。这可用于研究塔塔麦中继发代谢物的基因功能和产生,用于任何基因转化,或用于改良后的其他药用植物。
Tags
Hairy Roots
Agrobacterium Rhizogenes
Tartary Buckwheat
Fagopyrum Tataricum
Transformation
Seedling
Explant
Cotyledon
Hypocotyl
YEB Medium
Monoclonal Colony
Article
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