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Neuroscience
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从体外感觉和运动神经中纯化成纤维细胞和施万细胞
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro
Please note that all translations are automatically generated.
Click here for the English version.
从体外感觉和运动神经中纯化成纤维细胞和施万细胞
DOI:
10.3791/60952-v
•
08:16 min
•
May 20, 2020
•
Qianru He
,
Fanhui Yu
,
Yan Li
,
Junjie Sun
,
Fei Ding
1
Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair
,
Nantong University
Chapters
00:04
Introduction
00:45
Nerve Dissection and Cell Culture
02:32
Isolation and culture of Schwann Cells (SCs)
03:43
Isolation and Culture of Fibroblasts
05:27
Results: Morphology and Purity of Schwann Cells and Fibroblasts
07:48
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一种方法,从体外感觉和运动神经中纯化成纤维细胞和Schwann细胞。
Tags
Fibroblasts
Schwann Cells
Nerve Regeneration
Nervous System Diseases
Ventral Root
Dorsal Root
Trypsin
DMEM
FBS
PBS
Cell Culture
Cell Purification
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