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In Situ Detektion von Ribonucleoprotein Complex Assembly in der C. elegans Germline mit Proximity Ligation Assay
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay
DOI:

08:56 min

May 05, 2020

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Chapters

  • 00:04Introduction
  • 00:49Dissection/Gonad Extrusion
  • 02:46Fixation/Blocking
  • 04:14Primary Antibody Incubation and PLA Probe (Secondary Antibody) Incubation
  • 05:21Ligation
  • 06:09Amplification and Final Washes
  • 06:45Coverslip Mounting
  • 07:15Results: Germlines Following Co-immunostaining or PLA
  • 08:10Conclusion

Summary

Automatic Translation

Dieses Protokoll demonstriert die Verwendung des Proximity Ligation Assays zur Sonde für Protein-Protein-Wechselwirkungen in situ in der C. elegans Keimbahn.

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