Journal
/
/
毛利精密切肝片作为肝脏生物学的Ex Vivo模型
JoVE Journal
Medicine
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology
DOI:

12:36 min

March 14, 2020

, , , , , , , ,

Chapters

  • 00:00Introduction
  • 01:23Setup of the Vibratome
  • 03:15Liver Removal and Preparation
  • 06:07Production of Liver Slices
  • 10:14Results: Viability, Collagen, and Effects of Selected Bile Acids
  • 11:45Conclusion

Summary

Automatic Translation

该协议为从小鼠中生产可行的精确切肝片提供了一种简单可靠的方法。前活体组织样本可在实验室组织培养条件下维持多日,为检查肝病理生物学提供了灵活的模型。

Related Videos

Read Article