A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Hepatic Fibrosis Group,QIMR Berghofer Medical Research Institute, 2School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute,Curtin University, 3School of Veterinary and Life Sciences,Murdoch University, 4School of Medicine,The University of Queensland, 5Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC, 6School of Medicine,Griffith University, 7School of Biological Sciences,Queen’s University Belfast, 8Department of Gastroenterology & Hepatology,Fiona Stanley and Fremantle Hospital Group, 9School of Medical and Health Sciences,Edith Cowan University, 10Centre for Cell Therapy and Regenerative Medicine, School of Biomedical Sciences,University of Western Australia
Chapters
- 00:00Introduction
- 01:23Setup of the Vibratome
- 03:15Liver Removal and Preparation
- 06:07Production of Liver Slices
- 10:14Results: Viability, Collagen, and Effects of Selected Bile Acids
- 11:45Conclusion
Questo protocollo fornisce un metodo semplice e affidabile per la produzione di fette di fegato tagliate di precisione dai topi. I campioni di tessuto ex vivo possono essere mantenuti in condizioni di coltura dei tessuti di laboratorio per più giorni, fornendo un modello flessibile per esaminare la patobiologia epatica.
