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血管化、扩展发育和改进显微镜成像的肾器官和器官培养的优化

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Developmental Biology
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JoVE Journal Developmental Biology

Optimization of Renal Organoid and Organotypic Culture for Vascularization, Extended Development, and Improved Microscopy Imaging

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血管化、扩展发育和改进显微镜成像的肾器官和器官培养的优化

DOI:

10.3791/60995-v

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12:49 min

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March 28, 2020

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Susanna Kaisto, Ulla Saarela, Laura Dönges, Irina Raykhel, Ilya Skovorodkin*¹, Seppo J. Vainio*^1,2,3

¹Biocenter Oulu and Faculty of Biochemistry and Molecular Medicine,University of Oulu, ²InfoTech Oulu,University of Oulu, ³Biobank Borealis of Northern Finland,Oulu University Hospital

Chapters

00:00Introduction
02:00Protocol
08:11Results
11:56Conclusion

Summary

Automatic Translation

本文描述了研究器官发育的两种方法，一种是改善鸟类胚胎中胆囊膜（CAM）的异种移植设置，允许培养胚胎器官和器官的血管化，以及一种新颖的固定z方向器官培养方法具有改进的实验条件，允许高分辨率延时共聚焦成像。

Tags

Keywords: Renal Organoid Organotypic Culture Vascularization Extended Development Microscopy Imaging Kidney Organ Culture Stem Cells Kidney Development Blood Filtration Chorioallantoic Membrane Confocal Imaging Organ Culture Method Embryonic Kidneys Renal Organoids

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