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使用基于细胞的雌激素受体对植物雌激素进行筛查β记者分析
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay
DOI:

06:07 min

June 07, 2020

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Chapters

  • 00:04Introduction
  • 00:50Extraction of Potential Plant Phytosteroids
  • 02:29Human Estrogen Receptor β Transfection Assay
  • 04:25Results: ERβ Reporter Assay System for Phytoestrogen Activity Screening of Fruits and Vegetables
  • 05:19Conclusion

Summary

Automatic Translation

我们优化了一种市售雌激素受体β记者检测,用于筛查人类和非人类灵长类动物的食物,以进行雌激素活性活动。我们通过显示已知的雌激素人类食物大豆注册高,而其他食物显示没有活性来验证这一分析。

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