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Omvendt transkripsjon Loop-Mediert Isothermal Amplification (RT-LAMP) Analyse for spesifikk og rask deteksjon av Tilapia Lake Virus
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1Department of Biotechnology, Faculty of Applied Science,King Mongkut’s University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine,Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies,Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS),King Mongkut’s University of Technology North Bangkok (KMUTNB)
Chapters
- 00:03Introduction
- 00:59RNA Extraction
- 02:50Primer Design
- 05:20Demonstration of RT-Lamp Assay
- 07:10Results
- 08:19Conclusion
Vi presenterer en RT-LAMP analyse for påvisning av TiLV i tilapia fisk ved hjelp av enkle instrumenter over en relativt kort periode sammenlignet med konvensjonelle RT-PCR teknikker. Denne protokollen kan bidra til å kontrollere epidemispredningen av TiLVD, spesielt i utviklingsland.
