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Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish
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Developmental Biology
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JoVE Journal Developmental Biology
Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish

Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish

DOI:
10.3791/61035-v

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07:32 min

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April 29, 2021

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1Department of Biological Sciences,California State University, Chico

Chapters

  • 00:04Introduction
  • 00:3748 Hours Post Fertilization (HPF) Zebrafish Embryo Dechorionation
  • 01:42Dithiothreitol (DTT) Treatment
  • 02:17Embryo Dissociation
  • 03:13Flow Cytometric Sample Preparation
  • 03:51Flow Cytometry
  • 06:10Results: Representative Effects of Morpholino (MO)-Mediated Transcript Reduction on Red Blood Cell Production Numbers During Embryogenesis
  • 07:01Conclusion

Summary

Automatic Translation

Automatic Translation

This assay is a simple method to quantitate hematopoietic cells in developing embryonic zebrafish. Blood cells from dissociated zebrafish are subjected to flow cytometry analysis. This allows the detection of blood defects in mutant animals and after genetic modification.

Tags

Flow CytometryZebrafishBlood CellsEmbryonic DevelopmentGene KnockdownGene OverexpressionDissociationProteaseDTTDechorionation

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