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Chapters
- 00:04Introduction
- 00:42Short Tandem Target Mimic (STTM) Fragment Amplification
- 01:40STTM Purification
- 02:22T4 DNA Polymerase Reaction Mix Preparation
- 03:13Potato Virus X (PVX)-Based microRNA (miRNA) Silencing (VbMS) Vector Construction
- 04:55T4 DNA Polymerase Reaction Setup and STTM Cloning
- 07:21Potato Virus-X-Based VbMS Assay
- 10:07Results: Representative VbMS of miR165/166 in Tetraploid Potato Species
- 11:16Conclusion
Vi præsenterer en detaljeret protokol for kartoffelvirus X (PVX)-baserede microRNA silencing (VbMS) system til funktionelt karakterisere endogene mikroRNA'er (miRNAs) i kartoffel. Target mimic (TM) molekyler af miRNA af interesse er integreret i PVX vektor og forbigående udtrykt i kartoffel til tavshed målet miRNA eller miRNA familie.
