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人类多能干细胞和免疫损伤的成熟小脑有机体可伸缩生成
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining
DOI:

10:40 min

June 13, 2020

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Chapters

  • 00:04Introduction
  • 00:23Seeding the Bioreactor
  • 02:06Differentiation and Maturation of Aggregates
  • 03:26Preparing Organoids for Cryosectioning
  • 05:47Immunostaining
  • 08:27Results: Efficient Cerebellar Differentiation and Maturation
  • 10:09Conclusion

Summary

Automatic Translation

该协议描述了一个动态培养系统,用于利用一种一种用途的生物反应器,产生控制大小的人类多能干细胞,进一步刺激在化学定义和免饲料条件下小脑器官的分化。

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