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Journal
/
Bioengineering
/
人类多能干细胞和免疫损伤的成熟小脑有机体可伸缩生成
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Scalable Generation of Mature Cerebellar Organoids from Human Pluripotent Stem Cells and Characterization by Immunostaining
Please note that all translations are automatically generated.
Click here for the English version.
人类多能干细胞和免疫损伤的成熟小脑有机体可伸缩生成
DOI:
10.3791/61143-v
•
10:40 min
•
June 13, 2020
•
Teresa P. Silva
2
,
Tiago G. Fernandes
,
Diogo E. S. Nogueira
,
Carlos A. V. Rodrigues
,
Evguenia P. Bekman
2,3
,
Yas Hashimura
,
Sunghoon Jung
,
Brian Lee
,
Maria Carmo-Fonseca
,
Joaquim M. S. Cabral
1
iBB – Institute for Bioengineering and Biosciences and Department of Bioengineering, Instituto Superior Técnico
,
Universidade de Lisboa
,
2
Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina
,
Universidade de Lisboa
,
3
The Discoveries Centre for Regenerative and Precision Medicine, Lisbon Campus
,
Universidade de Lisboa
,
4
PBS Biotech, Inc, Camarillo, CA, USA
Chapters
00:04
Introduction
00:23
Seeding the Bioreactor
02:06
Differentiation and Maturation of Aggregates
03:26
Preparing Organoids for Cryosectioning
05:47
Immunostaining
08:27
Results: Efficient Cerebellar Differentiation and Maturation
10:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一个动态培养系统,用于利用一种一种用途的生物反应器,产生控制大小的人类多能干细胞,进一步刺激在化学定义和免饲料条件下小脑器官的分化。
Tags
Keywords: Cerebellar Organoids
Human Pluripotent Stem Cells
Scalable Generation
Single-use Bioreactors
Immunostaining
IPSC Aggregates
GfCDM Differentiation Medium
Cell Detachment
Centrifugation
Cell Counting
Bioreactor Cultivation
Medium Exchange
Organoid Storage
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