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在存在治疗性抗体的情况下,由FcγRIIIa参与驱动的人类自然杀伤细胞事件的评估
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies
DOI:

09:54 min

May 22, 2020

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Chapters

  • 00:05Introduction
  • 00:27Antibody-Mediated Activation of NK Cells
  • 03:12Interrogation of Signaling Molecules by Western Blot Analysis
  • 03:47Isolation of mRNA and Preparation for Chemokine/Cytokine Assessment
  • 04:39Cytoskeletal Rearrangement Assessment
  • 05:57Assessing Degranulation Using CD107a Surface Staining
  • 07:10Results: Downstream Applications of NK Cell Activation Assay
  • 09:20Conclusion

Summary

Automatic Translation

本文描述了通过人类自然杀伤细胞中的治疗性抗体研究FcγRIIIa驱动事件的方案。该人工刺激平台允许询问下游效应器功能,例如脱颗粒,趋化因子/细胞因子产生以及由参与结合的抗体的FcγRIIIa和Fc部分介导的信号通路。

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