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X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
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JoVE Journal Biochemistry
X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050

DOI:
10.3791/61288-v

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11:27 min

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May 13, 2020

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, , ,
1Laboratory of Microbiology, Department of Molecular Biology,Université Libre de Bruxelles, 2Labiris Institut de Recherche

Chapters

  • 00:04Introduction
  • 01:03Activity Assay
  • 02:19Apoenzyme Preparation
  • 03:32Dimer Preparation
  • 04:03Microseeding
  • 05:01X-Ray Diffraction
  • 05:48Indexation
  • 06:50Molecular Replacement
  • 08:04Model Building
  • 09:07Results: Representative Thermotoga Maritima M42 Aminopeptidase TmPep1050 Oligomeric State Transition
  • 10:50Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol has been developed to study the dimer-dodecamer transition of TmPep1050, an M42 aminopeptidase, at the structural level. It is a straightforward pipeline starting from protein purification to X-ray data processing. Crystallogenesis, data set indexation, and molecular replacement have been emphasized through a case of study, TmPep1050H60A H307A variant.

Tags

Keywords: X-ray CrystallographyOligomeric StateThermotoga MaritimaAminopeptidaseTmPep1050Activity AssayApoenzyme PreparationDialysisUltrafiltration

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