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JoVE Journal Genetics
Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction
 

Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

Article DOI: 10.3791/61383-v • 07:36 min • August 27th, 2020
August 27th, 2020 •
1, 1, 1, 1,2, 1, 1, 1, 3, 1
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University

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Summary
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We describe a method for evaluating the microRNA expression in the kidneys of mice with unilateral ureteral obstruction (UUO) by quantitative reverse-transcription polymerase chain reaction. This protocol is suitable for studying kidney microRNA expression profiles in mice with UUO and in the context of other pathological conditions.

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Keywords: MicroRNA Quantitative Real-time PCR Unilateral Ureteral Obstruction Renal Fibrosis Sham Surgery Ureteral Ligation Kidney Sample Homogenization Phenyl/guanidine-based Lysis Reagent
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