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A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants
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Genetics
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JoVE Journal Genetics
A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

DOI:
10.3791/59864-v

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06:34 min

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January 21, 2020

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, , ,
1Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry Sciences, 2State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agricultural Sciences and School of Life Sciences,Peking University

Chapters

  • 00:04Title
  • 01:15Installation and Testing
  • 02:56Identifying Novel MicroRNAs (miRNAs)
  • 03:33Reference Index Building
  • 04:42Results: Representative miRDP2 Performance
  • 05:45Conclusion

Summary

Automatic Translation

Automatic Translation

A bioinformatics pipeline, namely miRDeep-P2 (miRDP2 for short), with updated plant miRNA criteria and an overhauled algorithm, could accurately and efficiently analyze microRNA transcriptomes in plants, especially for species with complex and large genomes.

Tags

Bioinformatics PipelineMicroRNA TranscriptomesPlantsMiRDeep2Plant MicroRNAsTranscriptional RegulatorsPlant DevelopmentEnvironmental ChallengesFalse PositivesProcessing TimeHigh Confidence MicroRNA AnnotationGenome FunctionMiRDeep2 PipelineArabidopsis ThalianaGenome Reference IndexNoncoding RNA Reference IndexDeep Sequencing ReadsAdaptersFASTA Identifiers

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