A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:00Introduction
- 01:27Step 1: Preparation of a 15% Denaturing Polyacrylamide Gel
- 02:03Step 2: Assembly of the Glass Plates and Electrophresis Unit
- 04:13Step 3: Preparation of Loading Dye and Samples
- 05:38Step 4: Gel Electrophresis
- 06:33Step 5: Preparation for Electro-blotting
- 10:15Step 6: Preparation of Radiolabeled Probe
- 10:50Step 7: Hybridization of the Blot
- 12:36Representative Results
- 13:24Conclusion
