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基于细胞基和细胞外矩阵的Murine睾丸器官生成

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Developmental Biology

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JoVE Journal Developmental Biology

Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids

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基于细胞基和细胞外矩阵的Murine睾丸器官生成

DOI:

10.3791/61403-v

•

09:18 min

•

October 07, 2020

•

Maxwell E Edmonds, Micah D. Forshee, Teresa K. Woodruff

¹Department of Obstetrics and Gynecology, Feinberg School of Medicine,Northwestern University

Chapters

00:04Introduction
00:40Testis Tissue Dissociation
03:35Preparation of Organoid Culture Dishes and Seeding of Cells
06:29Results: Self Assembled Organoids in 2D and 3D, ECM and ECM-free Culture Conditions
08:38Conclusion

Summary

Automatic Translation

在这里，介绍了从原发性新生儿穆林睾丸细胞生成睾丸器官的四种方法，即细胞外基质（ECM）和无 ECM 的 2D 和 3D 培养环境。这些技术具有多种研究应用，特别适用于研究睾丸发育和体外生理学。

Tags

Testicular Organoids Extracellular Matrix 2D Culture 3D Culture Spermatogenesis Reproductive Endocrinology In Vitro Gametogenesis Mouse Dissection Testis Dissociation Enzymatic Digestion Cell Separation Cell Culture

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