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单肌纤维隔离与文化从埃默里-德雷福斯肌肉萎缩的早期后纳塔尔发展穆林模型
JoVE Journal
Medicine
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JoVE Journal Medicine
Single Myofiber Isolation and Culture from a Murine Model of Emery-Dreifuss Muscular Dystrophy in Early Post-Natal Development
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单肌纤维隔离与文化从埃默里-德雷福斯肌肉萎缩的早期后纳塔尔发展穆林模型

DOI:
10.3791/61516-v

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08:07 min

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July 01, 2020

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, , ,
1Istituto Nazionale di Genetica Molecolare “Romeo ed Enrica Invernizzi”, 2IRCCS Santa Lucia Foundation, 3Department of Biology and Biotechnology, “C. Darwin” Sapienza University,CNR – IBPM, 4CNR – Institute of Biomedical Technologies (ITB)

Chapters

  • 00:04Introduction
  • 00:55Muscle Dissection
  • 02:14Muscle Digestion
  • 02:48Single Myofiber Isolation and Culture
  • 03:54Immunofluorescence
  • 06:24Results: Representative Healthy Myofiber Isolation and Culture
  • 07:29Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们提出了一种方法，从同源突变Lamin+8-11小鼠模型，一个非常严重的模型Emery-Dreifus肌肉萎缩症（EDMD）在出生后早期发育阶段有效地获得单肌肉纤维。

Tags

Keywords: Single Myofiber IsolationEmery-Dreifuss Muscular DystrophyPostnatal DevelopmentMuscle Stem CellsMuscle DissectionMuscle DigestionMuscle Fiber IsolationWashing SolutionPetri Dish

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