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Journal
/
Immunology and Infection
/
フローサイトメトリーベースアッセイによる熱帯
熱マラリア原
虫に対する自然獲得食細胞症誘導抗体の測定
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Measuring Naturally Acquired Phagocytosis-Inducing Antibodies to
Plasmodium falciparum
Parasites by a Flow Cytometry-Based Assay
Please note that all translations are automatically generated.
Click here for the English version.
フローサイトメトリーベースアッセイによる熱帯
熱マラリア原
虫に対する自然獲得食細胞症誘導抗体の測定
DOI:
10.3791/61538-v
•
09:57 min
•
August 06, 2020
•
Maria del Pilar Quintana
,
Nsoh Godwin Anabire
2,3
,
Lars Hviid
4
1
Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences
,
University of Copenhagen
,
2
West African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology
,
University of Ghana
,
3
Department of Immunology
,
Noguchi Memorial Institute for Medical Research
,
4
Centre for Medical Parasitology, Department of Infectious Diseases
,
Rigshospitalet
Chapters
00:04
Introduction
00:33
THP-1 and Infected Erythrocyte (IE) Culture Setup
01:58
Parasite Staining and Opsonization Plate Preparation
03:47
THP-1 Cell and Phagocytosis Plate Preparation
04:35
Phagocytosis Assay
06:21
Flow Cytometry Acquisition and Analysis
07:34
Results: Representative Evaluation of Parasite-IE Opsonization and Phagocytosis
09:21
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルの全体的な目標は、自然に
熱帯熱マラリア原虫
感染にさらされた個人のセラまたは血漿中に存在する抗体の容量を測定する方法についての指示を提供し、寄生虫感染赤血球(IE)の貪食を誘導する。
Tags
Phagocytosis
Plasmodium Falciparum
Flow Cytometry
Antibodies
Parasites
Erythrocytes
THP-1 Cells
Parasitemia
Ethidium Bromide
Opsonization
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