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使用紫罗兰兴奋细胞渗透DNA结合染料分离穆林精子生成细胞
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye
DOI:

08:21 min

January 14, 2021

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Chapters

  • 00:04Introduction
  • 00:53Testicular Cell Suspension Preparation
  • 02:35Cell Staining
  • 03:30Flow Cytometry
  • 07:08Results: Representative Spermatogenic Cell Imaging, Yield, and Viability
  • 07:55Conclusion

Summary

Automatic Translation

在这里,我们提出了一个简单而有效的方法来从成年小鼠睾丸中分离活的美病细胞和后美生菌细胞。使用低细胞毒性、紫罗兰兴奋的DNA结合染料和荧光激活细胞分选,可以分离高度丰富的精子致原细胞群,用于许多下游应用。

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