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Isolatie van Murine Spermatogenic Cellen met behulp van een Violet-Excited Cell-Permeable DNA Binding Dye
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1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute,Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics,University of Cincinnati College of Medicine, 3Division of Germ Cell Biology, National Institute for Basic Biology,National Institutes of Natural Sciences, 4Department of Basic Biology, School of Life Science,Graduate University for Advanced Studies (Sokendai), 5Department of Animal Science and Biotechnology, School of Veterinary Medicine,Azabu University, 6Faculty of Science and Technology, Department of Applied Biological Science,Tokyo University of Science
Chapters
- 00:04Introduction
- 00:53Testicular Cell Suspension Preparation
- 02:35Cell Staining
- 03:30Flow Cytometry
- 07:08Results: Representative Spermatogenic Cell Imaging, Yield, and Viability
- 07:55Conclusion
Hier presenteren we een eenvoudige en efficiënte methode om levende meiotische en post-meiotische kiemcellen te isoleren van volwassen muispesten. Met behulp van een lage cytotoxiciteit, violet-opgewonden DNA bindende kleurstof en fluorescentie-geactiveerde celsorde, kan men zeer verrijkte spermatogene celpopulaties isoleren voor vele downstream toepassingen.
