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Journal
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Bioengineering
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实时显微镜成像下的 3D 水凝胶控制应变
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Controlled Strain of 3D Hydrogels under Live Microscopy Imaging
Please note that all translations are automatically generated.
Click here for the English version.
实时显微镜成像下的 3D 水凝胶控制应变
DOI:
10.3791/61671-v
•
07:41 min
•
December 04, 2020
•
Avraham Kolel
,
Avishy Roitblat Riba
,
Sari Natan
,
Oren Tchaicheeyan
,
Eilom Saias
,
Ayelet Lesman
4
1
Department of Biomedical Engineering, Faculty of Engineering
,
Tel-Aviv University
,
2
Department of Materials Science and Engineering, Faculty of Engineering
,
Tel-Aviv University
,
3
School of Mechanical Engineering, Faculty of Engineering
,
Tel-Aviv University
,
4
Center for the Physics and Chemistry of Living Systems
,
Tel-Aviv University
Chapters
00:04
Introduction
00:50
Silicone Strip Preparation
01:20
Fibrin Gel Polymerization
02:29
Sample Loading
03:42
Sample Adequacy Confirmation
04:26
Stretching Device Operation, Stretching and Imaging
05:42
Results: Representative Gel Strain Stretching and Gel Fiber Alignment Analysis
07:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
提出的方法涉及嵌入硅橡胶的3D软水凝胶的单轴拉伸,同时允许活体共焦显微镜。演示了外部和内部水凝胶菌株的特征以及纤维对齐。开发的设备和协议可以评估细胞对各种菌株机制的反应。
Tags
3D Hydrogels
Live Microscopy Imaging
Mechanical Cues
Biomechanical Responses
Hydrogel Geometry
Cell Response
Extracellular Matrix
Disease Progression
Cancer Therapies
Fibrin Gel
Fibrinogen
Thrombin
PBS
Stretching Device
Silicone Strip
Article
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