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实时显微镜成像下的 3D 水凝胶控制应变
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Controlled Strain of 3D Hydrogels under Live Microscopy Imaging
DOI:

07:41 min

December 04, 2020

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Chapters

  • 00:04Introduction
  • 00:50Silicone Strip Preparation
  • 01:20Fibrin Gel Polymerization
  • 02:29Sample Loading
  • 03:42Sample Adequacy Confirmation
  • 04:26Stretching Device Operation, Stretching and Imaging
  • 05:42Results: Representative Gel Strain Stretching and Gel Fiber Alignment Analysis
  • 07:02Conclusion

Summary

Automatic Translation

提出的方法涉及嵌入硅橡胶的3D软水凝胶的单轴拉伸,同时允许活体共焦显微镜。演示了外部和内部水凝胶菌株的特征以及纤维对齐。开发的设备和协议可以评估细胞对各种菌株机制的反应。

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