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1Department of Biomedical Engineering, Faculty of Engineering,Tel-Aviv University, 2Department of Materials Science and Engineering, Faculty of Engineering,Tel-Aviv University, 3School of Mechanical Engineering, Faculty of Engineering,Tel-Aviv University, 4Center for the Physics and Chemistry of Living Systems,Tel-Aviv University
Chapters
- 00:04Introduction
- 00:50Silicone Strip Preparation
- 01:20Fibrin Gel Polymerization
- 02:29Sample Loading
- 03:42Sample Adequacy Confirmation
- 04:26Stretching Device Operation, Stretching and Imaging
- 05:42Results: Representative Gel Strain Stretching and Gel Fiber Alignment Analysis
- 07:02Conclusion
Sunulan yöntem, canlı konfokal mikroskopiye izin verirken silikon kauçuğun içine gömülü 3D yumuşak hidrojellerin tek eksenli gerilmesini içerir. Dış ve iç hidrojel suşlarının karakterizasyonu ve fiber hizalaması gösterilmiştir. Geliştirilen cihaz ve protokol, hücrelerin çeşitli gerinim rejimlerine tepkisini değerlendirebilir.
