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大鼠骨骼肌中纤维-脂肪源性祖细胞（FAPs）和肌源性祖细胞（MPs）的鉴定、分离和表征
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JoVE Journal Biology
Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat
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大鼠骨骼肌中纤维-脂肪源性祖细胞（FAPs）和肌源性祖细胞（MPs）的鉴定、分离和表征

DOI:
10.3791/61750-v

•

09:49 min

•

June 09, 2021

•

, , ,
1Keenan Research Center for Biomedical Science,St Michaels Hospital, Unity Health Toronto, 2Department of Medicine and Interdepartmental Division of Critical Care Medicine,University of Toronto

Chapters

  • 00:04Introduction
  • 01:03Muscle Digestion
  • 02:28Generation of Single Cell Suspension
  • 04:06Antibody Staining for Flow Cytometry
  • 06:52Results: Identification of FAPs and MPs via Flow Cytometry
  • 09:20Conclusion

Summary

Automatic Translation

Automatic Translation

该协议概述了一种从大鼠骨骼肌中分离纤维脂肪源性祖细胞（FAPs）和肌源性祖细胞（MPs）的方法。在肌肉损伤模型中利用大鼠为分析提供了来自萎缩肌的组织可用性的增加，以及用于评估自由移动动物的肌肉力量和步态的更多经过验证的方法。

Tags

Fibro-Adipogenic Progenitors (FAPs)Myogenic Progenitors (MPs)Skeletal MuscleRatMuscle RegenerationFibrosisCollagenase IIDispaseCell Strainer

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