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Identifikation, isolation og karakterisering af fibro-adipogenic stamfaderer (FAPs) og myogene forfædre (parlamentsmedlemmer) i skeletmuskulatur i rotten
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JoVE Journal Biology
Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat

Identifikation, isolation og karakterisering af fibro-adipogenic stamfaderer (FAPs) og myogene forfædre (parlamentsmedlemmer) i skeletmuskulatur i rotten

DOI:
10.3791/61750-v

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09:49 min

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June 09, 2021

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, , ,
1Keenan Research Center for Biomedical Science,St Michaels Hospital, Unity Health Toronto, 2Department of Medicine and Interdepartmental Division of Critical Care Medicine,University of Toronto

Chapters

  • 00:04Introduction
  • 01:03Muscle Digestion
  • 02:28Generation of Single Cell Suspension
  • 04:06Antibody Staining for Flow Cytometry
  • 06:52Results: Identification of FAPs and MPs via Flow Cytometry
  • 09:20Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol skitserer en metode til at isolere Fibro-adipogenic forfædre (FAPs) og myogene forfædre (parlamentsmedlemmer) fra rotte skeletmuskulatur. Udnyttelse af rotten i muskelskade modeller giver øget væv tilgængelighed fra atrofisk muskel til analysen og et større repertoire af validerede metoder til at vurdere muskelstyrke og gangart i frit bevægende dyr.

Tags

Keywords: Fibro-Adipogenic Progenitors (FAPs)Myogenic Progenitors (MPs)Skeletal MuscleRatMuscle RegenerationFibrosisCollagenase IIDispaseCell Strainer

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