JoVE Journal
Biochemistry
Biochemistry
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Summary
Please note that all translations are automatically generated.
该协议描述了从过敏原中去除内源性脂质,并通过逆相HPLC与热退化用用户指定的配体替换它们。 31P-NMR 和圆形二分法允许快速确认配体去除/装载,并恢复原生过敏原结构。