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JoVE Journal
Biochemistry

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通过荧光体积排阻色谱快速评估膜蛋白质量
 
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通过荧光体积排阻色谱快速评估膜蛋白质量

Article DOI: 10.3791/64322-v 06:26 min January 6th, 2023
January 6th, 2023

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Summary

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Click here for the English version.

本协议描述了对膜蛋白进行荧光体积排阻色谱(FSEC)的程序,以评估其用于下游功能和结构分析的质量。介绍了在洗涤剂溶解和无洗涤剂条件下收集的几种G蛋白偶联受体(GPCR)的代表性FSEC结果。

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生物化学,第191期,
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