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A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch
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Engineering
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JoVE Journal Engineering
A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch

A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch

DOI:
10.3791/61824-v

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07:51 min

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December 10, 2020

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, , , , , , ,
1Department of Biomedical Engineering,Eindhoven University of Technology, 2Institute for Complex Molecular Systems (ICMS),Eindhoven University of Technology

Chapters

  • 00:04Introduction
  • 00:44Bioreactor Setup
  • 03:43Cell Seeding
  • 04:48Coupling Between the Bioreactor and the Pump System
  • 05:50Results: Representative Evaluation of the Biomaterial Inflammatory and Regenerative Capacity Under Flow and Stretch Conditions
  • 07:27Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to execute a dynamic co-culture of human macrophages and myofibroblasts in tubular electrospun scaffolds to investigate material-driven tissue regeneration, using a bioreactor which enables the decoupling of shear stress and cyclic stretch.

Tags

Keywords: BioreactorMulti-cueInflammatoryRegenerativeBiomaterialsFlowStretchHemodynamicsSheer StressCyclic StretchElectrospun ScaffoldSilicone TubingSuturePressure ConduitO-ringAdapter Bushing

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