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评估流和拉伸下生物材料的炎症和再生能力的多奎生物反应器

JoVE Journal
Engineering

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JoVE Journal Engineering

A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch

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评估流和拉伸下生物材料的炎症和再生能力的多奎生物反应器

DOI:

10.3791/61824-v

•

07:51 min

•

December 10, 2020

•

Suzanne E. Koch², Eline E. van Haaften², Tamar B. Wissing², Lizzy A. B. Cuypers, Jurgen A. Bulsink, Carlijn V. C. Bouten², Nicholas A. Kurniawan*^1,2, Anthal I. P. M. Smits*^1,2

¹Department of Biomedical Engineering,Eindhoven University of Technology, ²Institute for Complex Molecular Systems (ICMS),Eindhoven University of Technology

Chapters

00:04Introduction
00:44Bioreactor Setup
03:43Cell Seeding
04:48Coupling Between the Bioreactor and the Pump System
05:50Results: Representative Evaluation of the Biomaterial Inflammatory and Regenerative Capacity Under Flow and Stretch Conditions
07:27Conclusion

Summary

Automatic Translation

本协议的目标是在管状电喷脚手架中执行人类巨噬细胞和肌纤维细胞的动态共生，以研究物质驱动的组织再生，使用生物反应器，使剪切应力和循环拉伸脱钩。

Tags

Keywords: Bioreactor Multi-cue Inflammatory Regenerative Biomaterials Flow Stretch Hemodynamics Sheer Stress Cyclic Stretch Electrospun Scaffold Silicone Tubing Suture Pressure Conduit O-ring Adapter Bushing

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