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研究TGF-β信号和TGF-β引起的乳腺癌和正常细胞的上皮到中位过渡
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Cancer Research
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Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
Please note that all translations are automatically generated.
Click here for the English version.
研究TGF-β信号和TGF-β引起的乳腺癌和正常细胞的上皮到中位过渡
DOI:
10.3791/61830-v
•
06:54 min
•
October 27, 2020
•
Jing Zhang
,
Midory Thorikay
,
Gerard van der Zon
,
Maarten van Dinther
,
Peter ten Dijke
1
Oncode Institute and Department of Cell Chemical Biology
,
Leiden University Medical Center
Chapters
00:05
Introduction
01:57
Analysis of TGF-β-induced EMT
04:17
Results: TGF-β Signaling Responses
06:20
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们描述了一个系统的工作流程，通过研究这个信号通路中涉及的蛋白质和基因表达来研究TGF-β信号和TGF-β诱导的EMT。这些方法包括西方污渍、露西费拉塞记者检测、qPCR和免疫荧光染色。
Tags
TGF-beta
Epithelial-to-mesenchymal Transition (EMT)
Breast Cancer
NMuMG Cells
E-cadherin
F-actin
Cell Migration
Cell Invasion
Metastasis
Chemotherapy Resistance
Indirect Immunofluorescent Staining
Cell Density
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