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JoVE Journal Neuroscience
Microdissection of Mouse Brain into Functionally and Anatomically Different Regions
 

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

Article DOI: 10.3791/61941-v • 08:06 min • February 15th, 2021
February 15th, 2021 •
1, 1, 2, 3, 3, 2, 4, 2
1The Geneva Foundation, Medical Readiness Systems Biology, Walter Reed Army Institute of Research, 2Walter Reed Army Institute of Research, Medical Readiness Systems Biology, Center for Military Psychiatry and Neuroscience, 3ORISE Fellow, Medical Readiness Systems Biology, Walter Reed Army Institute of Research, 4Headquarter, Walter Reed Army Institute of Research

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Summary
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We present a hands-on, step-by-step, rapid protocol for mouse brain removal and dissection of discrete regions from fresh brain tissue. Obtaining brain regions for molecular analysis has become routine in many neuroscience labs. These brain regions are immediately frozen to obtain high quality transcriptomic data for system level analysis.

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Keywords: Mouse Brain Brain Dissection Brain Regions Molecular Analysis Anatomical Regions Structural Landmarks Microdissection Neuroscience Brain Anatomy
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