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初白和棕色预生细胞与新生小鼠的隔离和分化
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Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice
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初白和棕色预生细胞与新生小鼠的隔离和分化

DOI:
10.3791/62005-v

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09:00 min

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January 25, 2021

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, ,
1Department of Molecular Medicine,The Scripps Research Institute, La Jolla, CA, USA, 2Department of Medicine,University of Wisconsin-Madison, School of Medicine and Public Health, Madison, WI, USA

Chapters

  • 00:04Introduction
  • 01:32Adipose Depot Collection and Digestion
  • 03:28Plating Preadipocytes
  • 04:56Preadipocyte Culture Expansion
  • 06:17White and Brown Preadipocyte Differentiation
  • 06:55Results: Representative Adipocyte Progenitor Collection and Differentiation
  • 08:14Conclusion

Summary

Automatic Translation

Automatic Translation

本报告描述了一个同时将原生棕色和白色预生细胞与新生小鼠隔离的协议。孤立的细胞可以在培养中生长，并诱导分化成完全成熟的白色和棕色脂肪细胞。该方法使培养中原脂肪细胞的遗传、分子和功能特征化。

Tags

PreadipocytesAdipocyte IsolationAdipocyte DifferentiationNewborn MiceObesityDiabetesCell Culture Model

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