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斑马鱼卵巢卵巢中卵泡细胞和卵母细胞的分离

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Developmental Biology
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JoVE Journal Developmental Biology

Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish

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斑马鱼卵巢卵巢中卵泡细胞和卵母细胞的分离

DOI:

10.3791/62027-v

•

11:35 min

•

April 18, 2021

•

Wenyi Wang, Tao Kang, Lin Bai, Wei Hu, Yayoi Obata, Jianzhen Li

¹College of Life Sciences, Northwest Normal University, ²State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology, Chinese Academy of Sciences, ³Department of Bioscience,Tokyo University of Agriculture

Chapters

00:00Introduction
02:16Preparation of Pulled Glass Capillary
03:18Experimental Preparation
04:40Separation of Zebrafish Oocytes and Follicular Cells at Different Stages
08:08Results
10:12Conclusion

Summary

Automatic Translation

在这里，我们提出了一个简单的方法来分离斑马鱼卵泡中的卵泡细胞和卵母细胞，这将促进斑马鱼卵巢发育的调查。

Tags

Keywords: Follicular Cells Oocytes Ovarian Follicles Zebrafish Separation Dissection Glass Capillary In Vitro Fertilization Ovary Ovarian Development

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