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Analysis of Translation in the Developing Mouse Brain using Polysome Profiling
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Analysis of Translation in the Developing Mouse Brain using Polysome Profiling
DOI:

08:38 min

May 22, 2021

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Chapters

  • 00:04Introduction
  • 00:50Preparation of Sucrose Gradient
  • 01:57Tissue Dissection
  • 03:05Cell Lysis
  • 04:34Fractionation and Sample Collection
  • 06:15Results: Polysome Profiling Analysis of the Developing Mouse Cortex
  • 07:39Conclusion

Summary

Automatic Translation

The development of the mammalian brain requires proper control of gene expression at the level of translation. Here, we describe a polysome profiling system with an easy-to-assemble sucrose gradient-making and fractionation platform to assess the translational status of mRNAs in the developing brain.

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