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快速网格准备时间解决低温电子显微镜

JoVE Journal
Biochemistry
Author Produced

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JoVE Journal Biochemistry

Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy

Please note that all translations are automatically generated. Click here for the English version.

快速网格准备时间解决低温电子显微镜

DOI:

10.3791/62199-v

•

10:05 min

•

November 06, 2021

•

David P. Klebl, Frank Sobott, Howard D. White, Stephen P. Muench

¹School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology,University of Leeds, ²School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology,University of Leeds, ³Department of Physiological Sciences,Eastern Virginia Medical School

Chapters

00:10Introduction
00:43Preparing the System
04:34Fast Grid Preparation
07:12Time-Resolved Cryo-EM
08:46Representative Results
09:34Conclusion

Summary

Automatic Translation

在这里，我们提供了一个详细的协议，用于快速网格制作和快速混合和冻结的快速网格制作设备，以进行时间解决的实验。

Tags

Keywords: Cryo-electron Microscopy Time-resolved Grid Preparation Rapid Protein Concentration Syringe Pump Nitrogen Gas Buffer Spray Nozzle Plunging Arm

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