Journal
/
Developmental Biology
/
Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
JoVE Journal
Developmental Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

DOI:
10.3791/62272-v

•

06:53 min

•

July 10, 2021

•

, , , ,
1Department of Veterinary Medicine,University of Sassari

Chapters

  • 00:00Introduction
  • 01:11Protocol
  • 03:52Results
  • 05:38Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol aims at providing a standard method for the vitrification of adult and juvenile sheep oocytes. It includes all the steps from the preparation of the in vitro maturation media to the post-warming culture. Oocytes are vitrified at the MII stage using Cryotop to ensure the minimum essential volume.

Tags

Keywords: VitrificationOocyteIn Vitro MaturationSheepPrepubertalCryopreservationTrehaloseCryotopsWarmingCytoplasmZona PellucidaPolar BodyMinimum Essential Volume Method

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62272?article_type=v

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
check_url/62272?article_type=v

In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas
check_url/62272?article_type=v

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
check_url/62272?article_type=v

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
check_url/62272?article_type=v

Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
check_url/62272?article_type=v

Human Blastocyst Biopsy and Vitrification
check_url/62272?article_type=v

Minimum Volume Vitrification of Immature Feline Oocytes
check_url/62272?article_type=v

In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
check_url/62272?article_type=v

Accurate Follicle Enumeration in Adult Mouse Ovaries
check_url/62272?article_type=v

Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish

Read Article