/

/

低温结构照明微观数据收集来自低温保存细胞

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Please note that all translations are automatically generated. Click here for the English version.

低温结构照明微观数据收集来自低温保存细胞

DOI:

10.3791/62274-v

•

11:55 min

•

May 28, 2021

•

Nina Vyas*¹, Nina Perry*¹, Chidinma A. Okolo, Ilias Kounatidis, Thomas M. Fish, Kamal L. Nahas², Archana Jadhav, Mohamed A. Koronfel, Johannes Groen, Eva Pereiro, Ian M. Dobbie, Maria Harkiolaki

¹Harwell Science and Innovation Campus,Beamline B24, Diamond Light Source, ²Division of Virology, Department of Pathology,University of Cambridge, ³ALBA Synchrotron,Beamline 09 – MISTRAL, ⁴Micron Advanced Imaging Consortium, Department of Biochemistry,University of Oxford

Chapters

00:04Introduction
01:09Preparation of the Cryo-Stage
02:08Transfer of the Sample Storage Box into the Cryo-Stage
03:34Stage Docking and Focusing
05:03Brightfield Mosaic Acquisition and Identification of Areas of Interest
06:48Data Collection
08:59After Imaging
09:38Results: Cryo-structured Illumination Microscopic Imaging
11:00Conclusion

Summary

Automatic Translation

该协议演示了如何使用低温结构照明显微镜对生物低温保存样品进行成像。我们通过成像 U2OS 细胞的细胞骨骼来演示该方法。

Tags

Cryo-structured Illumination Microscopy Super Resolution Cryo Imaging Cellular Ultrastructure Vaccine Production Antibody Engineering Nano Particle Characterization Cryo-stage Liquid Nitrogen Sample Transfer Cassette TEM Grid

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

从美国的马蹄蟹，鲎波吕斐摩斯采血车

从环境中自然产生的趋磁细菌的采集，分离和富集

泄殖腔按摩与精子 DNA 分离法采集禽精

超结构保存细胞内膜蛋白定位电子显微镜分析的CryoAPEX方法

近植入阶段人类胚胎中特罗福细胞的单细胞集合

分离高质量鼠心房和心室肌细胞，同时测量 Ca²⁺ 瞬变和 L 型钙电流

蜜蜂群落花粉的采集和鉴定

从人骨关节炎膝关节收集和RNA提取

通过电子断层扫描对超构造保存的染色质中的复制结构域进行成像

冷冻电子断层扫描远程数据收集和断层扫描平均

Read Article