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Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint
 

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint

Article DOI: 10.3791/62718-v • 06:06 min • July 22nd, 2021
July 22nd, 2021 •
1, 1, 2, 1,3
1Bone and Joint Center, Henry Ford Health System, 2Department of Orthopedic Surgery, Henry Ford Health System, 3Center for Molecular Medicine and Genetics, Wayne State University

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Summary
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Primary tissues obtained from patients following total knee arthroplasty provide an experimental model for osteoarthritis research with maximal clinical translatability. This protocol describes how to identify, process, and isolate RNA from seven unique knee tissues to support mechanistic investigation in human osteoarthritis.

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Keywords: RNA Extraction Osteoarthritis Tissue Collection Human Knee Joint Gene Expression Histological Analysis Primary Human Tissues Cartilage Bone Meniscus Infrapatellar Fat Pad Anterior Cruciate Ligament Synovium Vastus Medialis Oblique Muscle Liquid Nitrogen Homogenization Mortar And Pestle Acid Guanidinium Phenol Solution
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